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. 2019 Jun 7;10:502. doi: 10.3389/fgene.2019.00502

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Copyright © 2019 Favia, Fitak, Guerra, Pierri, Faye, Oulmouden, Burger and Ciani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Myostatin protein expression. (A) Representative Western Blot of the eight considered dromedary muscles (1, deltoid; 2, extensor carpi radialis; 3, coccygeus; 4, biceps femoris; 5, peroneus longus; 6, semitendinosus; 7, tensor fasciae latae; 8, brachiocephalicus) performed using a rabbit polyclonal antibody (1:1000 dilution) that specifically binds the carboxy-terminal domain (Origene, TA343358). (B) Western Blot densitometric analysis of the promyostatin dimer (75 kDa), the promyostatin monomer (40 kDa) and the active C-terminal dimer (25 kDa), respectively. For each lane, optical density (OD) of the considered band is presented as a ratio over the total density of all the proteins transferred on the membrane and stained with Coomassie blue.