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. 2019 May 15;18(1):769–778. doi: 10.3892/etm.2019.7583

Figure 7.

Figure 7.

DKK3 knockdown reversed the inhibitory effects of miR-25 downregulation on glioma cell proliferation and migration. The (A) mRNA and (B) protein expression levels of DKK3 were determined by RT-qPCR and western blotting, respectively, in U251 and T98G cells following co-transfection with miR-25 inhibitor together with DKK3 siRNA or NC siRNA. Cell proliferation was examined by CCK-8 assays in (C) U251 and (D) T98G cells following transfection with miR-25 inhibitor and DKK3 siRNA or NC siRNA. Cell migration was examined using wound-healing assays in (E) U251 (magnification, ×200) and (F) T98G cells (magnification, ×40) following transfection with miR-25 inhibitor and DKK3 siRNA or NC siRNA. **P<0.01 vs. miR-25 in + siNC. DKK3, dickkopf WNT signaling pathway inhibitor 3; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; siRNA, small interfering RNA; NC, negative control; CCK-8, Cell Counting Kit-8.