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. 2019 May 20;18(1):425–434. doi: 10.3892/etm.2019.7595

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — LX2 cells are activated in a co-cultured system with THP-1 cells. (A) miR-152 expression in the LX2 cells during culture activation was detected by RT-qPCR. Data are presented as the mean ± standard deviation. (B) α-SMA mRNA expression in LX2 cells was analysed with RT-qPCR. Each value represents the mean ± SD of 3 experiments. (C) Albumin mRNA expression in LX2 cells was examined using RT-qPCR. Each value represents the mean ± SD of 3 experiments. (D) Western blot analysis of α-SMA and albumin in stimulated LX2 cells. The housekeeping protein GAPDH was used as loading controls for the western blot analysis and protein normalization. miR, microRNA; SD, standard deviation; RT-qPCR, reverse transcription quantitative polymerase chain reaction; α-SMA, α-smooth muscle actin; NC, negative control.