Skip to main content
. 2019 May 20;18(1):425–434. doi: 10.3892/etm.2019.7595

Table I.

Sequences of primers used for the RT-quantitative polymerase chain reaction assays.

miRNA/genes Primer sequences
miR-152 RT: 5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCCAAGTTC-3′
Forward: 5′-ACACTCCAGCTGGGTCAGTGCATGACAGAACT-3′
Reverse: 5′-CTCAACTGGTGTCGTGGA-3′
U6 RT: 5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAATATGG-3′
Forward: 5′-CTCGCTTCGGCAGCACA-3′
Reverse: 5′-AACGCTTCACGAATTTGCGT-3′
α-SMA (Human) Forward: 5′-GTTCCAGCCATCCTTCATCGG-3′
Reverse: 5′-CCTTCTGCATTCGGTCGGCAA-3′
Albumin (Human) Forward: 5′-CAGAATGCGCTATTAGTTCG-3′
Reverse: 5′-CTGGCGTTTTCTCATGCAA-3′
Gli3 (Human) Forward: 5′-TTTTCCCCTTTAATCTTGCCAT-3′
Reverse: 5′-CCAGTGGCAAATCAACCTCC-3′
β-actin (Human) Forward: 5′-CTCCATCCTGGCCTCGCTGT-3′
Reverse: 5′-GCTGTCACCTTCACCGTTCC-3′
α-SMA (Rat) Forward: 5′-GCGTGACTCACAACGTGCCTA-3′
Reverse: 5′-CCCATCAGGCAGTTCGTAGCTCT-3
Albumin (Rat) Forward: 5′-GATCTGCCCTCAATAGCTG-3′
Reverse: 5′-TGGCTTCATATTTCTTAGCAA-3′
Gli3 (Rat) Forward: 5′-CTCGACCATTTCCACGGCAAC-3′
Reverse: 5′-TCAGCACAGTGAAGTCTACACC-3′
β-actin (Rat) Forward: 5′-TCAGGTCATCACTATCGGCAAT-3′
Reverse: 5′-AAAGAAAGGGTGTAAAACGCA-3′

RT, reverse transcription; miR, microRNA; α-SMA, α-smooth muscle actin; Gli3, GLI family zinc finger 3.