Table I.
miRNA/genes | Primer sequences |
---|---|
miR-152 | RT: 5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCCAAGTTC-3′ |
Forward: 5′-ACACTCCAGCTGGGTCAGTGCATGACAGAACT-3′ | |
Reverse: 5′-CTCAACTGGTGTCGTGGA-3′ | |
U6 | RT: 5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAATATGG-3′ |
Forward: 5′-CTCGCTTCGGCAGCACA-3′ | |
Reverse: 5′-AACGCTTCACGAATTTGCGT-3′ | |
α-SMA (Human) | Forward: 5′-GTTCCAGCCATCCTTCATCGG-3′ |
Reverse: 5′-CCTTCTGCATTCGGTCGGCAA-3′ | |
Albumin (Human) | Forward: 5′-CAGAATGCGCTATTAGTTCG-3′ |
Reverse: 5′-CTGGCGTTTTCTCATGCAA-3′ | |
Gli3 (Human) | Forward: 5′-TTTTCCCCTTTAATCTTGCCAT-3′ |
Reverse: 5′-CCAGTGGCAAATCAACCTCC-3′ | |
β-actin (Human) | Forward: 5′-CTCCATCCTGGCCTCGCTGT-3′ |
Reverse: 5′-GCTGTCACCTTCACCGTTCC-3′ | |
α-SMA (Rat) | Forward: 5′-GCGTGACTCACAACGTGCCTA-3′ |
Reverse: 5′-CCCATCAGGCAGTTCGTAGCTCT-3 | |
Albumin (Rat) | Forward: 5′-GATCTGCCCTCAATAGCTG-3′ |
Reverse: 5′-TGGCTTCATATTTCTTAGCAA-3′ | |
Gli3 (Rat) | Forward: 5′-CTCGACCATTTCCACGGCAAC-3′ |
Reverse: 5′-TCAGCACAGTGAAGTCTACACC-3′ | |
β-actin (Rat) | Forward: 5′-TCAGGTCATCACTATCGGCAAT-3′ |
Reverse: 5′-AAAGAAAGGGTGTAAAACGCA-3′ |
RT, reverse transcription; miR, microRNA; α-SMA, α-smooth muscle actin; Gli3, GLI family zinc finger 3.