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. 2019 Jun 7;10:726. doi: 10.3389/fpls.2019.00726

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Copyright © 2019 Berleth, Berleth, Minges, Hänsch, Burkart, Stork, Stahl, Weidtkamp-Peters, Simon and Groth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MST based protein-protein interaction assay between AtCTR1 and receptor proteins AtETR1 and AtETR2. Binding of AtETR1 to fluorescently labeled AtCTR1 measured by MST resulted in a K_d value of 169(15) nM (○). For AtCTR1-AtETR2 complex formation a K_d value of 165(20) nM was obtained (●). As negative control, titration of chemically denatured AtCTR1 with AtETR2 is shown. Here, no binding event was observed (▲). Data are given as the mean (SD) of independent triplicates (●, ○) and duplicates (▲), respectively.