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. 2019 Jun 7;10:726. doi: 10.3389/fpls.2019.00726

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Copyright © 2019 Berleth, Berleth, Minges, Hänsch, Burkart, Stork, Stahl, Weidtkamp-Peters, Simon and Groth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intracellular localization of AtERS1 and AtERS2 transiently expressed in N. benthamiana epidermis cells. Confocal laser scanning microscopy images of mVenus (mV) and mCherry (mC)-tagged receptor proteins. (A–A″) AtERS2 does not colocalize with the PM dye FM4-64 and is instead found (B–B″) at the ER, where colocalization with the ER-mCherry marker protein is detected. AtERS2-mVenus colocalizes with (C–C″) AtERS1-mCherry, (D–D″) AtERS2-mCherry and (E–E″) BTI2-mCherry at the ER. (F–F″) AtERS2 tagged to mVenus and mCherry is also detected at the ER. Bars = 10 μm.