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. 2019 May 27;20(10):2592. doi: 10.3390/ijms20102592

Figure 1.

Figure 1

Treatment with the 30% DMSO Graptopetalum paraguayense (GP) extract, but not other extracts prepared from different solvents, suppressed Aurkb protein expression in HSC-T6 cells. (A) HSC-T6 cells were treated with GP extracts prepared from water, butanol, acetone, 70% methanol, 100% ethanol, 70% ethanol, 50% ethanol, 100% DMSO, and 30% DMSO at concentrations of 100 μg/mL, 150 μg/mL, 250 μg/mL, 500 μg/mL, 750 μg/mL, 1000 μg/mL, and 1500 μg/mL for 72 h, after which the cells were subjected to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays. * p < 0.05, ** p < 0.01, compared with the control group. (B) HSC-T6 cells were treated with the 30% DMSO GP extract at concentrations of 0 μg/mL, 250 μg/mL, 500 μg/mL, 750 μg/mL, and 1000 μg/mL for 24, 48, and 72 h, followed by MTT assays. $ p < 0.05, $$ p < 0.01, compared with the control group (24 h); # p < 0.05, ## p < 0.01, compared with the control group (48 h); * p < 0.05, ** p < 0.01, compared with the control group (72 h). (C) HSC-T6 cells were treated with GP extracts in H2O, acetone, 70% methanol, 100% ethanol, 70% ethanol, 50% ethanol, 100% DMSO, and 30% DMSO at 500 μg/mL for 24 h. The protein expression of Aurkb was detected by Western blot. 1: Control (H2O), 2: H2O extract, 3: Control (acetone), 4: acetone extract, 5: Control (methanol), 6: 70% methanol extract, 8: Control (ethanol), 9: 100% ethanol extract, 10: 70% ethanol extract, 9: 50% ethanol extract, 11: Control (DMSO), 12: 100% DMSO extract, 13: 30% DMSO extract. * p < 0.05, ** p < 0.01, compared with the control group.