Figure 4.

MC-1 mAb induces CCR5 endocytosis in CHO cells. CHO cells expressing CCR5 were incubated with MC-1 (▪]), MC-4 (□) (A), or RANTES (▪) (B) for 30 min at 37°C. Cell surface CCR5 was detected by flow cytometry with a saturating concentration of the same mAbs or MC-4 for RANTES-induced CCR5 down-modulation. Results were normalized for the fluorescence of unstimulated cells (100%) and for the background fluorescence (0%). All experiments were repeated at least twice with similar results.