Figure 2.

Effects of the tested compounds on cell viability of EBC-1 cancer cell line. (A) Percentage of viable cells (EBC-1) after 72 h exposure to the compounds at a concentration of 25 μM compared to the compound-free control received an equal volume of DMSO (100% viability). Each value was calculated from two independent experiments. The competitive inhibitory activity was expressed as an inhibition rate. (B) Growth inhibition effect of compounds 3d, 7a, 7b, 8a, 8b, 12c and 12d on EBC-1 cells. EBC-1 cells were seeded into 96-well cell culture plates and allowed to grow overnight. Thereafter, cells were treated with vehicle control (DMSO) or the compounds at the indicated concentration for 72 h. After treatment, 10 μL resazurin were added to the culture medium. After incubation at 37 °C for 5 h, fluorescence intensity was measured at a 530 nm excitation wavelength and a 590 nm emission wavelength using a Synergy HT photometer.