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. 2019 May 16;20(10):2436. doi: 10.3390/ijms20102436

Figure 7.

Figure 7

Green tea consumption increased glucose uptake and GLUT4 translocation in the skeletal muscle of KK-Ay mice. Mice were treated as shown in Figure 1B. On Day 63, the mice were orally administered glucose (2 g/kg body weight) and were sacrificed 1 h later. (A) Skeletal muscle was cut into small pieces of 50 mg each, and 3-OMG uptake was measured as described in “Materials and Methods”. The expressions of GLUT4 and β-actin in tissue lysates (B,C) and the quantities of GLUT4 and IRβ present in the plasma membrane fractions (D,E) were determined through western blot analysis, as described in “Materials and Methods”. In (B–E), the upper panels show typical immunoblots and the lower panels summarize the density of each band in all mice (n = 7). Data are expressed as the means ± SE. Values sharing the same letters were not significantly different (Tukey–Kramer multiple comparison test; p < 0.05).