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. 2019 May 16;20(10):2422. doi: 10.3390/ijms20102422

Figure 2.

Figure 2

The overexpression of CIPK11 increases cell damage caused by drought stress (A–C) The relative Proline, MDA, and H2O2 level in the Col-0, cipk11, CIPK11OE#1, and CIPK11OE#8 were calculated under normal and drought stress conditions. Error bars indicate SE (n = 3) and asterisks indicate significant differences from the Col-0 using the unpaired Student’s t-test (* p < 0.05; ** p < 0.001). (D) Detection of ROS by using H2DCFDA. Roots from Col-0, cipk11, CIPK11OE#1, and CIPK11OE#8 seedlings were subjected to liquid MS medium (Control) and exposed in room air for three minutes to simulate drought stress. The fluorescence of ROS (green) was imaged in the cells of root and the root images were collected after 15 min. incubation with H2DCFDA. Fluorescence indicates the presence of ROS.