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. 2019 May 21;20(10):2504. doi: 10.3390/ijms20102504

Figure 5.

Figure 5

Metabolic dysfunction induced AD-like pathology both in in vivo and in vitro model: (a,b) The expression levels of Aβ and BACE-1 were assessed by Western blot in palmitic acid-treated HT22 cells, and in HFD mice. β-actin was used as a loading control. For protein band quantification Image J software was used. One-way ANOVA followed by post-hoc analysis was used for statistical analysis. The density values were expressed in arbitrary units (AUs) as the mean ± SEM; (c) the expression level of neuronal synapse proteins (both pre-synapse, i.e., Synaptophysin and post-synapse density protein 95, i.e., PSD95) along with their respective histograms were analyzed through Western blot in the HFD-fed mice brain; (d) given are the representative images of double immunofluorescence staining of Aβ and BACE-1in Palmitic acid treated HT22 cells. The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01.