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. 2019 May 19;20(10):2471. doi: 10.3390/ijms20102471

Figure 2.

Figure 2

Schematic diagram of the symbiosis receptor-like kinase (VuSYMRK) gene structure, protein domains and the CRISPR-associated protein 9 (Cas9)/guide RNA (gRNA) binary vector. (A) Diagram of the VuSYMRK gene structure. The VuSYMRK gene is located on the reverse strand of chromosome 2 from 24313300 to 24322124. Grey line, untranslated region (UTR); black line, introns; grey box, exons; red arrows, gRNA target sites. The PAM sequences are in red font. (B) Diagram of predicted protein domains. SP, signal peptide; MLD, malectin-like domain; LRR, leucine-rich repeat; TM, transmembrane domain. (C) Illustration of the constructed binary vector for the CRISPR-Cas9 system. RB and LB, right and left borders; CaMV 35S pro, Cauliflower mosaic virus (CaMV) 35S promoter; CaMV 2X35S pro, CaMV 2X35S promoter; CaMV 35S term, CaMV 35S terminator; Nos term, nopaline synthase gene (Nos) terminator; NLS, nuclear localization sequence; LjU6 pro, L. japonicus U6 promoter.