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Results of computational alanine scanning (CAS) and experimental alanine scanning (EAS). (A) CAS, Pro441, Ile442, Asp443, Asn444, Asp446, Ile447, Thr448, and Phe450 had an energy of >0.5 kcal/mol after mutation to Ala; (B) EAS, the area under the curve (AUC) of the Pro441, Ile442, Asp443, Asn444, Ile447, and Thr448 is almost as with the negative control of Scramble control.
