Table 1.

Primers used for Slc7a11 RACE and cloning.

Name	Sequence (5′ to 3′)	Experiment
GSP1	CACTTTCTCCTGCCCA	5′RACE
GSP2	GGCTCCTTGCCACCCAT	5′RACE
GSP3	TCCCGTTCACGTTTCCC	5′RACE
C048-1	CCCTTTCCCTTTATTCGGACCCAT	3′RACE
C048-2	CGGGGTCCCTGCCTACTATCTCTT	3′RACE
Slc7a11 CDS-F	ATGGTCAGAAAACCTGTTGTGTCCACC	CDS amplification
Slc7a11 CDS-R	TTATTTACGACAGTCTTCTTCAGGTACA
Slc7a11-F	CCCAAGCTTATGGTCAGAAAACCTGTTGTGTCCACC	Vector construction
Slc7a11-R	TCCCCGCGGTTATTTACGACAGTCTTCTTCAGGTACA

Note: Bold letters indicate restriction enzyme cutting sites, italics indicate protective base.