Table 6.

EMSA reaction system.

Ingredient	1	2	3	4	5	6
PolydI:dC/μL	0.5	0.5	0.5	0.5	0.5	0.5
10× Binding Buffer/μL	0.0	0.0	1.5	1.5	1.5	1.5
Nuclear protein/μL	0.0	0.0	2.0	2.0	2.0	2.0
Biotin-labeled normal probe/μL	0.5	0.0	0.5	0.0	0.0	0.0
Biotin-labeled mutant probe/μL	0.0	0.5	0.0	0.5	0.0	0.0
Positive nuclear extract NF-KB	0.0	0.0	0.0	0.0	0.5	0.0
Negative nuclear extract NF-KB	0.0	0.0	0.0	0.0	0.0	0.5
ddH2O/μL	14.5	14.5	11.5	11.5	11	11