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Cold-competitive EMSA reaction system.
| Ingredient | 1 | 2 | 3 | 4 | 5 |
|---|---|---|---|---|---|
| Unlabeled probe/labeled probe | 40:1 | 80:1 | 40:1 | 80:1 | |
| 10× Binding Buffer/μL | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Nuclear protein/μL | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| Biotin-labeled normal probe/μL | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Unlabeled normal probe | 0.0 | 1.0 | 3.0 | 0.0 | 0.0 |
| Unlabeled mutant probe | 0.0 | 0.0 | 0.0 | 1.0 | 3.0 |
| ddH2O/μL | 11.0 | 10.0 | 8.0 | 10.0 | 8.0 |
