Figure 1.

Morphological and phenotypic characterization of adipose-derived stromal cell (ADSC) cultures. (A) Cell morphology (first panel) and adipogenic and osteogenic differentiation of ADSCs (second and third panels, respectively) at 40× magnification using phase-contrast microscopy. Scale bar = 30 µM; (B) immunophenotyping of ADSCs by flow cytometry; peaks are the expression of the selected molecules (black trace), compared to the negative isotype control (red trace); (C) curve of ADSC in vitro growth over time; (D) S100B protein content in ADSCs and adipose tissue, as measured by ELISA. Representative data of 6 independent experiments performed in triplicate.