Table 1.
Data on identification by MS and MS/MS methods of proteins corresponding to the first peak after gel filtration of exo-1 (Figure 5B) and exo-3 (Figure 6A) and their following separation by SDS-PAGE.
| Protein Number | Database MM (Da) a | Identified Proteins b | |
|---|---|---|---|
| exo-1 | exo-3 | ||
| 1 | 1 | 15988 or 15248 | Hemoglobin subunit alpha and beta |
| 2 | 20007 | Ferritin c | |
| 3 | 2 | 25673 | CD63 |
| 4 | 3 | 25809 | CD81 |
| 4 | 35914 | Annexin A5 | |
| 5 | 38580 | Annexin A2 | |
| 6 | 41710 or 41766 | Actin cytoplasmic | |
| 5 | 7 | 57341 or 57917 | Alkaline phosphatase |
| 6 | 8 | 69321 | HAS c |
| 9 | 77014 | Serotransferrin | |
| 7 | 10 | 104788 | Alpha-actinin-4 |
| 8 | 11 | 150–170 kDa | IgG H and L chains c |
a MS—determination of a set of peptides from tryptic hydrolysates by MS/MS in accordance with the sequences of peptides (from three to seven peptides). b For the identification of proteins and their molecular masses, the 2016 SwissProt program was used. c Unlike other proteins, ferritin, HSA, and IgGs are absent in exosome preparations after their trypsin treatment; apparently, these proteins are not internal exosome proteins.