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. 2019 May 16;20(10):2431. doi: 10.3390/ijms20102431

Figure 1.

Figure 1

Downregulation of Kr-h1 partially recovered the deteriorating effect of E93 knock-down on metamorphosis. (A) External genitalia phenotype of brown planthoppers after RNAi of E93 and Kr-h1 alone or together. Transmission electron microscopy (TEM) was used to observe the phenotype of external genitalia after the eclosion of adults. Nymphs were injected at the 4th instar stage. (B) dsRNAs of E93 and Kr-h1 were injected alone or together at the 3rd instar larval stage, and the survival rate after moulting from the 3rd to 4th instar nymph stage is shown; (C) dsRNA of E93 and Kr-h1 were injected alone or together at the 4th instar nymph stage, survival rate after moulting from 4th instar nymph to 5th instar nymph is shown; (D) survival rate after metamorphosis from the 5th instar nymph to adult after the injection of E93 and Kr-h1 alone or together. Duncan’s multiple comparison was used, and different letters indicate significant differences (p < 0.05).