Figure 3.

Electron microscopy analysis and quantification of Lon^VQ dodecamer formation. Negative stain electron micrographs of Lon^WT (left) and Lon^VQ (right). Under the same experimental conditions and upon immobilization on the grids, Lon^VQ forms a higher percentage of dodecamers. Representative dodecamer and hexamer particles are denoted with white and black arrows, respectively. Inset left: representative class images of dodecamers (top) and hexamers (bottom) based on the Lon^WT negative stain particles.20 Inset right: zoomed in view of raw Lon^VQ particles. The three particles on the left in white boxes are dodecamers and the particle on the right in the black box is a representative hexamer. Scale bar shown represents 100 nm.