Figure 5.

In vivo degradation of IbpA by Lon upon heat shock. Representative Western blots of E. coli cell extracts during heat shock (45°C) monitoring loss of the IbpA protein over time. Bottom. Protein bands were quantified and the percentage of IbpA protein remaining when Lon^WT (circles), Lon^VQ (squares), or Δlon (triangles) was expressed is plotted as a function of time. The experiment was performed in triplicate and error bars represent SEM (lines connecting data points are not indicative of statistically based fitting).