Figure 3.

The treatment with CXCL14 induces the phosphorylation of ERK1 and ERK2 in U87MG cells. Representative Western blot showing total (ERK1/2) (lower panel) and phosphorylated (phospho-ERK1/2) ERK1/2 (upper panel) proteins in total protein extracts of MCF7 or U87MG cells treated or not with recombinant human CXCL14 (400 ng/mL). The graphs show the densitometric quantification of the detected bands, and represent the average (+/− st. dev.) of three independent experiments. The panel relative to MCF7 cells, assayed as positive controls of CXCL14 action on ERK phosphorylation, was produced after a longer exposure, in order to reveal the faint bands present in untreated cells. * p < 0.05.