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. 2019 May 26;20(10):2580. doi: 10.3390/ijms20102580

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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qRT-PCR analysis of PREF1 gene expression in freshly isolated hASCs at passage 4 (hASCs CNT), hASCs lentivirally transducted with scrambled (negative) and shRNA against NANOG (hASCs sh NANOG). PREF1 was significantly downregulated in hASCs sh NANOG. Relative expression levels were assessed using the 2^−ΔΔCt method. Data are representative of three independent experiments with the fold change compared to expression levels in a commercial human adipose stem cell line (ASC52telo, hTERT immortalized adipose-derived mesenchymal stem cell). The experiment was repeated at least three times ** p < 0.01.