Cytotoxicity of nanoparticles (NPs). Cytotoxicity of (A) CuO, (B) Cu, (C) ZnO nano, (D) Mix γ-Fe2O3/Fe3O4, (E) Mix γ-Fe2O3/Fe3O4-citrate, (F) Mix γ-Fe2O3/Fe3O4-malate, (G) ZnO micro, (H) SiO2, (I) SiO2-COOH, (J) SiO2-NH2, (K) C60, (L) γ-Fe2O3, (M) γ-Fe2O3+SiO2, (N) γ-Fe2O3+SiO2-NH2, (O) γ-Fe2O3+SiO2-COOH. Cytotoxicity of NPs to A549 cells was evaluated by the MTT assay, after a 24-h exposure. Results are expressed as mean (+SD) percentage of untreated control cells in a representative experiment performed twice in five replica wells. Asterisk indicates a significant difference with respect to untreated control cells (dashed line; * equals p < 0.05; ** equals p < 0.01; *** equals p < 0.001; one-way ANOVA, Bonferroni’s post hoc test).