Figure 1.

Hyperglycemia in STZ-induced diabetic rats is associated with an increased proliferation rate but decreased osteogenic differentiation and matrix mineralization of primary bone marrow stromal cells (BMSCs). (A) Blood glucose levels and (B) HbAlc levels of normoglycemic (control) and diabetic (STZ) rats. Primary BMSCs from control and diabetic rats were harvested, and their cell proliferation rates were determined using the XTT assay (C), COL1 and ALP expression levels in BMSCs from control and diabetic rats were determined using western blotting (D), and matrix mineralization in BMSCs was determined using the ARS staining quantification assay (E). Data are represented as mean ± SEM from three to four independent experiments. *, P < 0.05 vs. control cells. **, P < 0.001 vs. control cells.