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. 2019 May 10;16(5):696–703. doi: 10.7150/ijms.32707

Figure 2.

Figure 2

High glucose treatment presents a dose-dependent inhibitory effect on osteogenic differentiation and matrix mineralization of mesenchymal progenitors. RMSC-bm cells were used as control (5.5 mM), or stimulated with high glucose (15, 25, and 35 mM) for 7 and 14 days. Western blotting was used to determine COL1 and ALP expression levels in RMSC-bm cells (A), β-actin used as an internal control. The ARS staining quantification assay was used to define the extent of matrix mineralization in RMSC-bm cells (B). Data are represented as mean ± SEM from three to four independent experiments. *, P < 0.05 vs. control cells.