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. 2019 May 10;16(5):696–703. doi: 10.7150/ijms.32707

Figure 4.

Figure 4

Activation of the Notch2 signaling pathway might play a role in high glucose-induced inhibition of osteogenic differentiation. RMSC-bm cells were used as control (5.5 mM), or stimulated with high glucose (15, 25, and 35 mM) for 2 and 7 days. Western blotting was used to determine Notch1, Notch2, and ALP expression levels in RMSC-bm cells (A), β-actin used as an internal control. RMSC-bm cells were used as control (50 µg/mL), or stimulated with high AGE (100 and 200 µg/mL) for 2 days. Western blotting was used to determine Notch2 and ALP expression levels (B), β-actin used as an internal control. Data are represented as mean ± SEM from three to four independent experiments. rs, Spearman's rank correlation coefficient. *, P < 0.05 vs. control cells. **, P < 0.001 vs. control cells.