Figure 1.

Inhibition of the WE(2-1) and AL(2-1) on LPS-induced nitric oxide (NO) in RAW 264.7 macrophages. (A) RAW 264.7 cells (1 × 10⁴ cells/well) were treated with the indicated concentrations of various extracts for 24 h and cell viability was determined by MTT assay. Results of independent experiments were averaged and are shown as percentage cell viability compared with the viability of untreated control cells. (B) The nitrite production was measured by the Griess reaction assay method as described in the methods section. Cells were pre-treated with indicated concentrations of various extracts for 2 h and stimulated with LPS (1 µg/mL) for 22 h. The values obtained were compared with those of standard concentrations of sodium nitrite dissolved in RPMI 1640 medium, and the concentrations of nitrite in a conditioned media of sample treated cells were calculated. Data were obtained from three independent experiments and were expressed as means ± SD. ***p < 0.001 indicates significant differences from the unstimulated control group.