Figure 4.

WE(2-1) and AL(2-1) stimulates filaggrin and serine palmitoyltransferase (SPT). (A) HaCaT cells (1 × 10⁶ cells/well) were exposed to indicated concentrations of WE(2-1) and AL(2-1) for 24 h, and then supernatant collected for investigation using a Hyaluronic acid assay kit following the manufacturer’s instructions. (B) HaCaT cells (1 × 10⁶ cells/well) were treated with the indicated concentrations of WE(2-1) and AL(2-1) for 24 h. Total RNA was isolated, and filaggrin and SPT mRNA expressions were examined by RT-PCR analysis. PCR of glyceraldehydes-3-phosphatedehydrogenase, GAPDH, was performed to control for a similar initial cDNA content of the sample. The results shown are representative of the three independent experiments. (C) HaCaT cells (1 × 10⁶ cells/well) were treated with indicated concentrations of WE(2-1) and AL(2-1) for 24 h and then analyzed by Western blot analysis using antibodies against filaggrin, and SPT. β-actin was used as a loading control. The blots shown are representative of three independent experiments that had similar results.