Figure 4.

miR-27a-5p attenuates hypoxia-induced excessive autophagy and apoptosis in H9c2 cells. H9c2 cells were exposed to hypoxia for 24 h after transfection of a specific miR-27a -5p mimics or inhibitor. Transfection efficiency was analyzed by qRT-PCR (A). Cell viability (B), membrane damage (C), and cell apoptosis (D–F) were assessed by CCK8 assays, LDH release assays, apoptosis staining (scale bar: 50 μm), flow cytometry and qRT-PCR analysis, respectively. The level of autophagy was evaluated by GFP-LC3 fluorescence after hypoxia for 24 h (G); scale bar: 5 μm. Autophagy-related proteins were detected by western blot (H). Three independent experiments were performed in triplicate. Data are expressed as the mean ± SD. * p < 0.05, ** p < 0.01. NC: negative control.