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. 2019 May 27;20(10):2605. doi: 10.3390/ijms20102605

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Schematic representation of a size-exclusion chromatography multi-angle light scattering (SEC-MALS) system. The buffer is de-gassed and pumped into the system. The sample is loaded into the system and carried through the size-exclusion chromatography (SEC) column where the size separation process takes place before the different components of the sample passes through the three detectors: a ultraviolet (UV) detector, a multi-angle light scattering (MALS) detector, and a refractive index (RI) detector. The detectors’ output signals are combined and analysed by the available software.