Enhancers vs Promoters
A) Enhancers function independent of orientation. If an enhancer is flipped, it maintains its gene regulation function. A flipped promoter, however, will block gene transcription.
B) Enhancers function independent of location. An enhancer that is moved downstream or upstream remains functional. A promoter that is no longer directly adjacent to the TSS will not drive transcription.
Teaching point: Enhancers function independent of orientation and location, unlike promoters.