Skip to main content
. 2019 May 27;20(10):2601. doi: 10.3390/ijms20102601

Figure 4.

Figure 4

Oxidized purines-specific mtDNA damage at the D-loop, Ori-L, and DR1 regions in liver from 28- and 32-month-old rats. (A) Bars represent the mean and SEM of the values obtained for each of the five animals analyzed in both groups. Bars not showing the same superscript differ significantly; p < 0.05, Dunn’s Multiple comparison test after Kruskal-Wallis test. (B) Representative gel of formamidopyrimidine DNA glycosylase (Fpg)-treated and untreated total DNA from a 28-month-old rat and a 32-month-old animal; 2.5 and 5 ng total DNA were amplified using the D-loop long primer set (Table 1). An aliquot of each PCR amplification was loaded onto agarose ethidium bromide-stained gel and analyzed for band intensities (a: molecular weight marker (GeneRuler 100 bp DNA Ladder, Thermo Fisher Scientific); b, c, e, f, h, i, m, and n: 2.5 ng total DNA as PCR template; d, g, l, and o: 5 ng total DNA as PCR template).