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. 2019 May 23;20(10):2528. doi: 10.3390/ijms20102528

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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A validation of breast TMA with tumor cores from 145 patients. (A) MALDI-IMS of this TMA showing representative deceased (green circles) and survived (red circles) patients from stage 1 and 2 samples. Quantitation of each group shows that tumor samples from deceased patients had statistically significant higher levels of F(6)A4G4Lac1 than those from survived patients (p = 0.01). (B) Patients with the higher signal of tetra-antennary with one lactosamine arm and one fucose (F(6)A4G4Lac1) correlate with higher risk and poor survival (measured in months). (C) Analysis of RNA expression of FUT8 from TCGA shows higher levels of FUT8 in cancer vs. normal patients. **** p < 0.0001.