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. 2019 May 27;20(10):2594. doi: 10.3390/ijms20102594

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The flowchart of the transformation system for P. alba × P. glandulosa. (A) Pre-culture of leaf explants on M1. (B) Shoot induction of leaf explants on M3 after co-cultivated with Agrobacterium bacteria. (C) Shoot induction of leaf explants on M3 for 4 weeks. (D) Shoots cultured on M4 for root induction. (E) Root induction on M4 for 2 weeks. (F) Transgenic poplar seedlings culture on M4.