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. 2019 May 27;20(10):2594. doi: 10.3390/ijms20102594

Figure 3.

Figure 3

PCR, qRT-PCR, and GUS staining analysis of transgenic poplars. (A) PCR product of 35S-GUS with 680 bp was detected in Atu, and all of the transgenic poplars, PCR product of actin gene with 250 bp was detected in all of the poplars, and PCR product of tzs gene with 660 bp was detected in Atu. M, Marker; Atu, Agrobacterium tumefaciens; WT, wild type of P. alba × P. glandulosa; 1–20, transgenic poplars. (B) GUS staining of different transgenic poplar and WT plants. WT, wild type; Line 1–Line 7, different lines of transgenic poplars. Bars = 0.5 cm. (C) GUS staining of different explants of Line 6. TB terminal bud; L, leaf; JS, juvenile stem; AS, adult stem; R, root. Bars = 0.5 cm. (D) qRT-PCR analysis of transgenic poplar and WT plants. WT, wild type; Line 1–Line 7, different lines of transgenic poplars.