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Conditions of high-performance liquid chromatography (HPLC) for analyzing sugars and organic acids.
| Conditions | Sugars | Organic Acid |
|---|---|---|
| Column | YMC-Pack Polyamine II (250 × 4.6 mm) | YMC-Triart C18 (3 µm, 12 nm), 150 × 3.0 mm |
| Eluent | Acetonitrile/water (75/25, v/v) | 20 mM H3PO4 |
| Oven temp. | 26 °C | 37 °C |
| Flow rate | 1.0 mL/min | 1.0 mL/min |
| Detector | RI (Shodex RI-101, Tokyo, Japan) | UV at 220 nm |
| Inj. Volume | 20 µL | 20 µL |
