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. 2019 May 20;20(10):2481. doi: 10.3390/ijms20102481

Figure 3.

Figure 3

The effects of phloretin on mineralization and expression of osteoblastogenic and adipogenic markers in MC3T3-E1 cells. (A,B) After reaching confluence, MC3T3-E1 cells were incubated in osteoblast differentiation medium with 0, 10, and 50 µM phloretin, and von Kossa staining, Alizarin red staining, and its quantification were performed on day 14. Quantification results are expressed as mean ± SE (n = 6). *** p < 0.001. (C–L) After reaching confluence, MC3T3-E1 cells were incubated in osteoblast differentiation medium with 0 and 50 µM phloretin. The mRNA expression of osteoblast differentiation markers (Alp, Col-1, Ocn, Runx2, and Osx) and adipocyte differentiation markers (Pparγ, C/ebpα, Fas, Fabp4, and Apn) was examined on day 3 by real-time PCR. The results are expressed as mean ± SE (n ≥ 5). *** p < 0.001. Phl; phloretin.