Figure 2.

Alignment of plasmids recovered from strain Sa21 using Easyfig (A) and the BLAST Ring Image Generator (BRIG) (B). Plasmid pSa21-TC-CIP was used as a reference in (B); representative genes are labeled. S. Agona strain, Sa21, harbours two plasmids, pSa21-HP and pSa21-CIP. The two plasmids were fused during transconjugation and formed a single plasmid pSa21-TC-CIP. The PMQR gene qnrS in plasmid pSa21-CIP was incorporated into the new plasmid pSa21-TC-CIP, which became conjugative. Plasmid sequence was generated through combination of both Illumina and PacBio sequencing data.