Figure 4.

Alignment of plasmids recovered from strain Sa27 using Easyfig (A) and the BLAST Ring Image Generator (BRIG) (B); representative genes are labeled. S. Derby strain, Sa27, harboured two plasmids, pSa27-CIP and pSa27-HP. The two plasmids were fused during conjugation process and formed a single plasmid pSa27-TC-CIP. Antimicrobial resistance genes qnrS, bla_TEM, oqxA, oqxB, sul2 and tetA in plasmid pSa27-CIP were integrated to form the new plasmid pSa27-TC-CIP, which became conjugative. Plasmid sequence was generated through combination of both Illumina and PacBio sequencing data.