Figure 2.

Western blot analysis of Nrf2, NQO1 and TKT after 24 h of treatment of HeLa cells with the indicated compounds. Compounds 9e and 9f were used at the concentration of 10 µM, whereas 9g was used at the concentration of 5 µM due to its limited solubility in phosphate buffer. t-BHQ was used at the concentration of 50 µM as a positive control. To confirm equal protein loading, each membrane was stripped and reprobed with anti-β-actin antibody.