Figure 5.

GLE inhibits Lpd expression in MDA-MB-231 breast cancer cells. MDA-MB-231 (1.4 × 10⁴) cells were grown in 5% FBS media for 24 h prior to treatment with vehicle or GLE (0.25 mg/mL, 0.96 mg/mL) for 24 h. Cells were fixed and stained with DAPI (blue) and anti-Lamellipodin (green) per manufacturer’s instructions. White arrows show lamellipodia. Experiments represent data obtained from three independent biological replicates. Zoom in each image is shown in white squares, and each share equal dimensions. Scale bars = 30 μm.