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. 2019 Jun 9;34(1):1158–1163. doi: 10.1080/14756366.2019.1624541

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The results of Western blotting key ROS stress proteins and CPUL1 docked into the binding site of the TrxR1. (A) Western blotting results of ASK1, p-ASK1, p38, p-p38, p-JNK and JNK after treated by different doses of CPUL1 (2, 4 and 8 μM). Curcumin used as positive control. (B–I) The levels of proteins in (A) were normalized to β-actin. Values represent the mean ±SD obtained from three different experiments. *p < 0.05, **p < 0.01, ***p < 0.001 significantly different from the value of control (untreated). (J) Total view of CPUL1 was docked into the binding site of the TrxR1. (K) Detailed view of CPUL1 was docked into the binding site of the TrxR1.