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. 2019 Jun 3;34(1):1140–1151. doi: 10.1080/14756366.2019.1621863

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — SI-4650 induced Autophagy. (A) A549 cells were treated for 48 h with SI-4650 (80 μM), along with DMSO as control. Levels of LC3 and p62 were analysed by western blotting. (B) A549 cells incubated without or with 80 μM SI-4650 for 48 h were imaged by transmission electron microscopy. (C) The effects of SI-4650 on A549 cells with GFP-LC3. A549 cells were transfected with pEGFP-LC3 plasmid and then treated with SI-4650 (80 μM) or DMSO for 48 h. Autophagy spot aggregation in A549 cells with GFP-LC3 was pictured by laser scanning confocal microscopy. Scale bar, 100 μm.