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. 2019 Jun 9;8(1):879–894. doi: 10.1080/22221751.2019.1625728

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Cyclin D1 transcriptional inhibiting NTCP is p53 dependent. (A) NTCP promoter activity was tested by dual luciferase reporter system. Cyclin D1-T286A plasmid was co-transfected with wild type NTCP promoter or NTCP promoter containing the mutant p53 binding site (Figure 5(F)). (B) The protein level of p53 in SMMC7721 and Huh-7 after transfected with cyclinD1-T286A plasmid were detected by western blot. (C) Luciferase reporter assays were conducted to analyse the effect of cyclin D1-T286A on the activity of NTCP promoter containing the mutant E2F binding site. (D) ChIP-PCR assay of E2F1 not binding to the highly conserved NTCP promoter in Huh-7 cells. (*P < 0.05; **P < 0.01; ***P < 0.001; ns: not significant).