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. 2019 May 27;20(10):2591. doi: 10.3390/ijms20102591

Figure 4.

Figure 4

Osteogenic and hypertrophic differentiation processes in rAAV-transduced hMSC aggregates. Aggregates were co-transduced with rAAV TGF-β/IGF-I, TGF-β/lacZ, IGF-I/lacZ or transduced with rAAV lacZ as described in Figure 1, Figure 2 and Figure 3 and in the Materials and Methods. Samples were processed after 21 days to evaluate the deposition of type-I and -X collagen (immunohistochemistry) and matrix mineralization (alizarin red staining) (magnification ×10; all representative data). Scale bars: 100 µm.