Table 1.
Real-time PCR primer sequences and siRNA sequences.
| Gene | Forward (5′ → 3′) | Reverse (5′ → 3′) |
|---|---|---|
| Mouse GAPDH | GCACAGTCAAGGCCGAGAAT | GCCTTCTCCATGGTGGTGAA |
| Mouse FGF21 | CQTCTGCCTCAGAAGGACTC | AAGGCTCTACCATGCTCAGG |
| Rat β-actin | TTTAATGTCACGCACGATTTC | CCCATCTATGAGGGTTACGC |
| Rat FGF21 | AGATCAGGGAGGACGGAACA | TCAGGATCAAAGTGAGGCGAT |
| Negative control siRNA | UUCUCCGAACGUGUCACGUTT; ACGUGACACGUUCGGAGAATT |
|
| Rat siRNA-FGF21 (#1) | CAACCAGAUGGAACUCUCUAUGGAU; AUCCAUAGAGAGUUCCAUCUGGUUG |
|
| Rat siRNA-FGF21 (#2) | GCAGUUUCAGAGAGCUGCUGCUUAA; UUAAGCAGCAGCUCUCUGAAACUGC |
|
| Rat siRNA-FGF21 (#3) | CCCUGAGCAUGGUAGAGCCUUUGCA; UGCAAAGGCUCUACCAUGCUCAGGG |