Table 1. C. showae strains characterized in this study.

Strain	Patient	Patient type	Sample type	Diagnosis	Country of origin	Sequencing Platform^	Adhesion to colonic epithelial cells+	Invasion of colonic epithelial cells*
B32_SW	B32	Paediatric	Biopsy	Crohn’s	UK	IM + PB	4.85 ± 8.08	0.17 ± 0.03
B91_SC	B91	Paediatric	Biopsy	Crohn’s	UK	IM + PB	0.15 ± 0.68	0.00 ± 0.00
B91_SCBr	B91	Paediatric	Biopsy	Crohn’s	UK	IM	0.50 ± 1.08	0.00 ± 0.00
B91_TPP	B91	Paediatric	Biopsy	Crohn’s	UK	IM	0.27 ± 0.86	0.00 ± 0.00
129_MSG	129	Adult	Biopsy	Adenoma	UK	IM + PB	3.42 ± 8.12	0.10 ± 0.01
129_VTPP	129	Adult	Biopsy	Adenoma	UK	IM	4.68 ± 9.78	0.13 ± 0.03
129_VTPPs	129	Adult	Biopsy	Adenoma	UK	IM	2.45 ± 3.04	0.11 ± 0.01
CAM	CAM	Adult	Stool	Gastroenteritis	Denmark	PB	1.34 ± 2.72	0.33 ± 0.07

Note: All strains’ isolation location was colonic, and all strains were positive in the Vero cell line cytotoxicity assay. Results for adhesion and invasion assays are mean ± SD of independent experiments.

^IM (Illumina MiSeq), PB (PacBio-RSII).

⁺Mean number of bacteria ± SD per HT-29 cell after 6 h incubation.

*Mean percentage ± SD of the original inoculum after 1 h gentamicin treatment of 6h-infected HT-29 cells.