Table 1. C. showae strains characterized in this study.
Strain | Patient | Patient type | Sample type | Diagnosis | Country of origin | Sequencing Platform^ | Adhesion to colonic epithelial cells+ | Invasion of colonic epithelial cells* |
---|---|---|---|---|---|---|---|---|
B32_SW | B32 | Paediatric | Biopsy | Crohn’s | UK | IM + PB | 4.85 ± 8.08 | 0.17 ± 0.03 |
B91_SC | B91 | Paediatric | Biopsy | Crohn’s | UK | IM + PB | 0.15 ± 0.68 | 0.00 ± 0.00 |
B91_SCBr | B91 | Paediatric | Biopsy | Crohn’s | UK | IM | 0.50 ± 1.08 | 0.00 ± 0.00 |
B91_TPP | B91 | Paediatric | Biopsy | Crohn’s | UK | IM | 0.27 ± 0.86 | 0.00 ± 0.00 |
129_MSG | 129 | Adult | Biopsy | Adenoma | UK | IM + PB | 3.42 ± 8.12 | 0.10 ± 0.01 |
129_VTPP | 129 | Adult | Biopsy | Adenoma | UK | IM | 4.68 ± 9.78 | 0.13 ± 0.03 |
129_VTPPs | 129 | Adult | Biopsy | Adenoma | UK | IM | 2.45 ± 3.04 | 0.11 ± 0.01 |
CAM | CAM | Adult | Stool | Gastroenteritis | Denmark | PB | 1.34 ± 2.72 | 0.33 ± 0.07 |
Note: All strains’ isolation location was colonic, and all strains were positive in the Vero cell line cytotoxicity assay. Results for adhesion and invasion assays are mean ± SD of independent experiments.
^IM (Illumina MiSeq), PB (PacBio-RSII).
+Mean number of bacteria ± SD per HT-29 cell after 6 h incubation.
*Mean percentage ± SD of the original inoculum after 1 h gentamicin treatment of 6h-infected HT-29 cells.