Figure 1.
The resveratrol rice DJ526 callus extended the lifespan of Drosophila melanogaster. (A) ORR male, (B) ORR female, (C) Harwich male, and (D) Harwich female. Ctrl indicates the standard cornmeal media of Drosophila; RES (resveratrol) indicates the standard cornmeal diet supplemented with 5692.42 µg/L resveratrol, the equivalent amount of resveratrol content in DJ526 media; DJ indicates the diet in which 50% of cornmeal was replaced with the Dongjin callus; and DJ526 indicates the diet in which 50% of cornmeal was replaced with the DJ526 callus (Table S1). All comparisons were made using Log-rank tests. p values (Log-rank tests) for each strain and each sex were as follows: (A) ORR male flies: DJ526 versus Ctrl (p = 0.0064), DJ526 versus RES (p = 0.0284), DJ526 versus DJ (p = 0.0803), Ctrl versus RES (p = 0.4824). (B) ORR female flies: DJ526 versus Ctrl (p = 0.0236), DJ526 versus RES (p = 0.0656), DJ526 versus DJ (p = 0.3446), Ctrl versus RES (p = 0.4380). (C) Harwich male flies: DJ526 versus Ctrl (p < 0.0001), DJ526 versus RES (p < 0.0001), DJ526 versus DJ (p = 0.0375), Ctrl versus RES (p = 0.3857). (D) Harwich female flies: DJ526 versus Ctrl (p = 0.0002), DJ526 versus RES (p = 0.0033), DJ526 versus DJ (p = 0.2959), Ctrl versus RES (p = 0.2610). Percentage survival is shown along with the maximum lifespan in each group. For the lifespan assays, the survival rate of 150 flies from each group was observed with media changed every 4 days until all flies were dead.